Genetic Toxicology
In BRF, the Genetic Toxicology Laboratory was designed to the International standards as per the GLP compliance. The Scientists in the department are well experienced and in-depth knowledge in the field of Genetic Toxicology.
Genetic Toxicology describes the assessment of the deleterious effects induced by physical or chemical agents in the hereditary material and related genetic process of living cells, which may lead to cancer. The alteration of genes can have direct or indirect effects of the DNA that induce single and double strand breaks, loss of excision repair, crosslinks, alkali labile sites, point mutations & structural and numerical aberrations.
The mutagenic and genotoxic effect of the various chemicals are evaluated by the series of experiments listed below. These experiments were conducted as per the National (CIB) and International guidelines (OECD, OPPTS / OCSPP and ISO).
| Bacterial reverse mutation assay (Ames test) using Salmonella typhimurium |
| Mammalian chromosome aberration test using Cell line |
| Mammalian cell micronucleus test using Cell line |
| Mouse lymphoma assay using cell line |
| Skin Corrosion: Re-constructed Human Epidermis (RHE) Test Method |
| Mutagenicity evaluation of Invitro mammalian cell gene mutation test (HPRT) |
| Skin Corrosion: |
| Mammalian erythrocyte micronucleus test using mice / rat |
| Mammalian bone marrow chromosome aberration test using mice / rat |
| Mammalian alkaline comet assay |
